AP®︎ Biology: Topic 3.4 Practice Test
Prepare for your quiz, test, or the AP exam with focused practice questions on Topic 3.4 of AP Biology – Photosynthesis.
Questions List
Topic 3.4
Question 1 Easy
This question tests the following: ENE-1.H
What is the primary source of energy for most living organisms?
What You’re Being Tested On:
Explore the learning objectives taken directly from the College Board’s AP® Biology Curriculum. Ensure you’re prepared for the exact topics covered on the AP® exam, in-class tests, and quizzes, and gain confidence in your mastery of the material.
Topic 3.4: Photosynthesis
Learning Objective: 3.4.A
Describe the photosynthetic processes and structural features of the chloroplast that allow organisms to capture and store energy.
Essential Knowledge: 3.4.A.1
Photosynthesis is the series of reactions that use carbon dioxide CO₂, water (H₂O), and light energy to make carbohydrates and oxygen (O₂). i. Photosynthetic organisms capture energy from the sun and produce sugars that can be used in biological processes or stored. ii. Photosynthesis first evolved in prokaryotic organisms. iii. Scientific evidence supports the claim that prokaryotic (cyanobacterial) photosynthesis was responsible for the production of an oxygenated atmosphere. iv. Prokaryotic photosynthetic pathways were the foundation of eukaryotic photosynthesis. Exclusion: Memorization of the steps in the Calvin cycle, the structure of the molecules, and the names of the enzymes involved, with the exception of ATP synthase, is beyond the scope of the AP Exam.
Essential Knowledge: 3.4.A.2
Stroma and thylakoids are found within the chloroplast. i. The stroma is the fluid within the inner chloroplast membrane and outside the thylakoid. The carbon fixation (Calvin cycle) reactions of photosynthesis occur in the stroma. ii. The thylakoid membranes contain chlorophyll pigments organized into two photosystems, as well as electron transport proteins. iii. Thylakoids are organized in stacks called grana. The light reactions of photosynthesis occur in the grana.
Essential Knowledge: 3.4.A.3
The light reactions of photosynthesis in eukaryotes involve a series of coordinated reaction pathways that capture energy present in light to yield ATP and NADPH, which power the production of organic molecules in the Calvin cycle. This provides energy for metabolic processes.
Learning Objective: 3.4.B
Explain how cells capture energy from light and transfer it to biological molecules for storage and use.
Essential Knowledge: 3.4.B.1
Electron transport chain (ETC) reactions occur in chloroplasts, in mitochondria, and across prokaryotic plasma membranes. In photosynthesis, electrons that pass through the thylakoid membrane are picked up and ultimately transferred to NADP⁺, reducing it to NADPH in photosystem I. Exclusion: The full names of the specific electron carriers in the electron transport chain are beyond the scope of the AP Exam. Exclusion: Specific steps, names of enzymes, and intermediates of the pathways for these processes are beyond the scope of this course and the AP Exam.
Essential Knowledge: 3.4.B.2
During photosynthesis, chlorophylls absorb energy from light, boosting electrons to a higher energy level in photosystems I and II. Water then splits, supplying electrons to replace those lost from photosystem II.
Essential Knowledge: 3.4.B.3
Photosystems I and II are embedded in the thylakoid membranes of chloroplasts and are connected by the transfer of electrons through an ETC.
Essential Knowledge: 3.4.B.4
When electrons are transferred between molecules in a series of oxidation/reduction reactions as they pass through the ETC, an electrochemical gradient of protons (hydrogen ions) is established across the thylakoid membrane. The membrane separates a region of low proton concentration outside the thylakoid membrane from a region of high proton concentration inside the thylakoid membrane.
Essential Knowledge: 3.4.B.5
The formation of the proton gradient is linked to the synthesis of ATP from ADP and inorganic phosphate via ATP synthase. The flow of protons back through membrane-bound ATP synthase by chemiosmosis drives the formation of ATP from ADP and inorganic phosphate; this is known as photophosphorylation.
Essential Knowledge: 3.4.B.6
The energy captured in the light reactions and transferred to ATP and NADPH powers the production of carbohydrates from carbon dioxide in the Calvin cycle. This occurs in the stroma of the chloroplast.
