AP®︎ Biology: Topic 6.8 Practice Test
Prepare for your quiz, test, or the AP exam with focused practice questions on Topic 6.8 of AP Biology – Biotechnology.
Questions List
Topic 6.8
Question 1 Easy
This question tests the following: IST-1.P
Which technique is used to amplify DNA sequences?
What You’re Being Tested On:
Explore the learning objectives taken directly from the College Board’s AP® Biology Curriculum. Ensure you’re prepared for the exact topics covered on the AP® exam, in-class tests, and quizzes, and gain confidence in your mastery of the material.

Topic 6.8: Biotechnology
Learning Objective: 6.8.A
Explain the use of genetic engineering techniques in analyzing or manipulating DNA.
Essential Knowledge: 6.8.A.1
Genetic engineering techniques can be used to analyze and manipulate DNA and RNA. i. Gel electrophoresis is a process that separates DNA fragments by size and charge. ii. During polymerase chain reaction (PCR), DNA fragments are amplified by denaturing DNA, annealing primers to the original strand, and extending the new DNA molecule. iii. Bacterial transformation introduces foreign DNA into bacterial cells. iv. DNA sequencing technology determines the order of nucleotides in a DNA molecule. Typically, these techniques result in a DNA fingerprint that allows for the comparison of DNA sequences from various samples. Exclusion: Knowledge of the details of each of these genetic engineering techniques is beyond the scope of the AP Exam. Illustrative examples: Amplified DNA fragments can be used to identify organisms and perform phylogenetic analysis; Analysis of DNA can be used for forensic identification; Genetically modified organisms include transgenic animals; Gene cloning allows propagation of DNA fragments.